With the goal of ending the AIDS epidemic by 2030, the Government of Ghana has adopted UNAIDS’ 90-90-90 targets for its HIV programme. To inform the government of resource needs, HP+ conducted a public sector costing and policy analysis of Ghana’s HIV programme clinical cascade. The analysis determined the additional costs for scaling up HIV services to meet adult and paediatric targets, explored the possibility for cost efficiencies that can be achieved from differentiated care service delivery, and considered costs for interventions to help retain and reinitiate clients that have been lost to follow-up along the clinical cascade. Findings from this study can inform resource allocation decisions that will allow the government to achieve its ambitious scale-up targets more effectively. They may also be used to advocate for financing and to inform future policy and programme decisions across the clinical cascade in Ghana.